Examples:

import hashlib

BUF_SIZE = 65536

md5 = hashlib.md5()

with open('path/to/file', 'rb') as fd_in:
    while True:
        data = fd_in.read(BUF_SIZE)
        if not data:
            break

        md5.update(data)

print('md5: {}'.format(md5.hexdigest()))
# md5: f94a4f3c13d2b75e266226f3644c9a01

References:

1. https://docs.python.org/3/library/hashlib.html